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KMID : 0613820180280050605
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Journal of Life Science
2018 Volume.28 No. 5 p.605 ~ p.614
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Safety of Nano-sized Bee Pollen in both In-vitro and In-vivo Models
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Pyeon Hae-In
So Soo-Jeong
Bak Ji-A
Lee Seung-Hyun
Lee Seung-Min
Suh Hwa-Jin
Lim Je-Oh
Kim Jung-Woo
Kim Sun-Youn
Lee Se-Ra
Lee Yong-Hyun
Chung Il-Kyung
Choi Yun-Sik
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Abstract
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Bee pollen has an outer wall which is resistant to both acidic and basic solutions and even the digestive enzymes in the gastrointestinal tract. Therefore, the oral bioavailability of bee pollen is only 10-15%. A previous study reported on wet-grinding technology which increased the extraction of active ingredients from bee pollen by 11 times. This study was designed to investigate the safety of wet- ground bee pollen. First, a single dose of wet-ground bee pollen was tested in both rats and beagle dogs at dosages of 5, 10, and 20 g/kg and 1.5, 3, and 6 g/kg, respectively. In rats, compound-colored stools were found in those administered 10 g/kg or more of wet-ground bee pollen. In beagle dogs, 6 g/kg of wet-ground bee pollen induced diarrhea in one male for four hours. However, no obvious clinical signs were found through the end of the experiment in rats and beagle dogs. In addition, no histological abnormality was found in all animals. The data indicates that a single dose of up to 20 g/kg of wet-ground bee pollen is safe. Next, the genetic toxicity of nano-sized bee pollen was tested. This study employed a bacterial reverse mutation test, a micronucleus assay, and a chromosomal aberration assay. In the micronucleus assay, there was no genetic toxicity up to the dosage of 2 g/kg. There was also no genetic toxicity in the bacterial reverse mutation test and chromosomal aberration assay. This data provides important information in developing nano-sized bee pollen into more advanced functional foods and herbal medicines.
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KEYWORD
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Bacterial reverse mutation, bee pollen, chromosomal aberration, micronucleus assay, single dose toxicity
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